| 1. | Research on the component of fibrinolytic activity in bacterial fermented douchi
细菌型豆豉中溶栓活性成分的初步研究 |
| 2. | The fibrinolytic activity in leukemic cell lines and its alteration on all - trans retinoic acid treatment
2细胞耐药转运分子作用的研究 |
| 3. | Effects of carvedilol on fibrinolytic activity and insulin resistance in patients with essential hypertension
卡维地洛对高血压病患者纤溶活性及胰岛素抵抗的影响 |
| 4. | Studies on screening , identification and fermentation characteristics of new natto - producing strain with the strong fibrinolytic activity
豆豉纤溶酶产生菌发酵条件研究 |
| 5. | The expressed proteins accumulated up to 40 % of bacterial soluble protein after iptg induction and formed inclusion bodies , which have no fibrinolytic activity
表达量都高达菌体可溶性蛋白的40 ，主要以包涵体的形式存在，没有检测到纤溶活性。 |
| 6. | With the incision of tumors and recovery from operation trauma , anticoagulant activity and fibrinolytic activity of the patients gradually returned to normal level . conclusion : coagulation disorders in laryngeal cancer patients had existed in a way
结果：患者术前即存在抗凝活性明显降低，并伴有纤溶活性增强，手术创伤使上述异常进一步加重。 |
| 7. | As a serine proteases , the expression product was detected with a relatively high protease activity . a similar result was also obtained from fibrin plate assay , it reveal ed that the expressed nk was provided with fibrinolytic activity
由于纳豆激酶是一种蛋白酶，我们首先检测并发现表达产物具有蛋白酶活性，通过纤维蛋白平板检测表达产物具有溶圈活性，表明表达的纳豆激酶具有溶解血栓的生物学功能。 |
| 8. | By using fibrin plate screening , fibrinolytic activity assay , sds - page analysis and thrombolytic effect test in vitro , two bacterial strains ( dc - 2 and dc - 4 ) producing strongly fibrinolytic enzymes were successfully isolated from douchi , a traditional chinese fermented - soybean food
利用纤维蛋白平板筛选、纤溶活性测定和体外溶栓实验相结合的方法，成功地从豆豉中筛选到2株纤溶活性较高和具有良好体外溶栓效果的细菌菌株dc - 2和dc - 4 。 |
| 9. | The recombinant pcr technic was used to introduce a linking peptide klgggg to the site between scfv single chain form of the monoclonal antibody sz - 51 specific for the glycoprotein gmp140 on activated platelet membrane and uk32 low molecular weight form of pro - urokinase , to make the scfv - linker - uk32 chimeric gene . this gene was cloned into the transfer vector pbacpak9 , and cotransfected with bacpak6 bsu36i digest into sf 9 cells . the fusion protein was secreted into the medium . in the fifth day after the cotransfection , the supernatant of the medium showed 107 iu ml fibrinolytic activity , higher than 25 iu ml fibrinolytic activity of scfv - uk32 . elisa showed that the supernatant had the binding activity to activated platelet . wastern blotting also indicated that the supernatant could bind to the monoclonal antibody of urokinase b chain
为了提高重组导向溶栓分子scfv - uk32的溶纤活性，通过重组pcr方法在编码scfv与uk32的碱基之间引入编码klgggg连接肽的碱基序列，并克隆到转移载体pbacpak9上,通过与线性病毒dna bacpak6 bsu36i digest共转染到昆虫细胞sf 9内，进行表达。表达产物分泌到上清中，共转染后第5d天用纤维平板法测得sf 9细胞上清溶纤活性达到107 iu ml ，比未引入连接肽的scfv - uk32的表达活性25 iu ml高。 |
| 10. | Under these conditions the fibrinolytic activity of the supernatant can reach 820 urokinase units per milliliter broth . ba - dfe was purified from the supernatant of b . amyloiquefaciens dc - 4 culture broth by ammonium sulfate precipitation , ion - exchange chromatography on cm - and deae - sepharose fast flow , hydrophobic interaction chromatography on phenyl sepharose 6 fast flow and gel filtration on sephadex g - 50 . the purified enzyme displayed thermophilic , hydrophilic and strong fibrinolytic activity
通过硫酸铵分级沉淀、 cm - sepharosefastflow和deae - sepharosefastflow离子交换层析、 phenylsepharose6fastflow疏水层析和sephadexg - 50凝胶过滤等方法，从解淀粉芽孢杆菌dc - 4的发酵液中分离纯化出电泳纯的ba - dfe 。 |
